Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general.

@article{Zhang2014PredictingDL,
  title={Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general.},
  author={Shiyun Zhang and Alexa Garcia-D'Angeli and J. Patrick Brennan and Qun Huo},
  journal={The Analyst},
  year={2014},
  volume={139 2},
  pages={439-45}
}
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the… CONTINUE READING
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