Predicted structural alterations in proinsulin during its interactions with prohormone convertases.

@article{Lipkind1999PredictedSA,
  title={Predicted structural alterations in proinsulin during its interactions with prohormone convertases.},
  author={Gregory M. Lipkind and Donald F. Steiner},
  journal={Biochemistry},
  year={1999},
  volume={38 3},
  pages={890-6}
}
The intracellular conversion of proinsulin to insulin occurs via cleavage at the two dibasic sites: Arg31-Arg32, B chain-C-peptide (BC) junction; and Lys64-Arg65, A chain-C-peptide (CA) junction, catalyzed by the subtilisin-like prohormone convertases SPC3 (PC1/PC3) and SPC2 (PC2), respectively. In this report we propose a possible conformational variant of proinsulin that would facilitate the formation of enzyme-substrate complexes at the BC and AC junctions of proinsulin with the substrate… CONTINUE READING