Predictable mosaic transgene expression in ascidian embryos produced with a simple electroporation device.

@article{Zeller2006PredictableMT,
  title={Predictable mosaic transgene expression in ascidian embryos produced with a simple electroporation device.},
  author={Robert W. Zeller and Michael J. Virata and Angela C. Cone},
  journal={Developmental dynamics : an official publication of the American Association of Anatomists},
  year={2006},
  volume={235 7},
  pages={
          1921-32
        }
}
Two customized electroporators were specifically designed for creating transgenic ascidian embryos. These electroporators were simple to build, inexpensive, and produced transgenic embryos with efficiencies that equaled or rivaled commercially available machines. A key design feature of these machines resulted in the generation of consistent electroporation pulses providing repeatability between experiments. These devices were used to optimize experimental parameters allowing for the creation… CONTINUE READING

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