Precursors of glutamic acid nitrogen in primary neuronal cultures: Studies with15N

Abstract

We utilized gas chromatography-mass spectrometry to study the transfer of15N from [2-15N]glutamine, [15N]leucine, [15N]alanine, or15NH4Cl to [15N]glutamate and [15N]aspartate in cultured cerebrocortical GABA-ergic neurons from the mouse. Initial rates of15N appearance (atom % excess) were somewhat higher with 2mM [2-15N]glutamine as a precursor than with… (More)
DOI: 10.1007/BF01208579

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@article{Yudkoff1990PrecursorsOG, title={Precursors of glutamic acid nitrogen in primary neuronal cultures: Studies with15N}, author={Marc Yudkoff and Itzhak Nissim and Leif Hertz}, journal={Neurochemical Research}, year={1990}, volume={15}, pages={1191-1196} }