Precursor-product discrimination by La protein during tRNA metabolism

  title={Precursor-product discrimination by La protein during tRNA metabolism},
  author={M. A. Bayfield and R. Maraia},
  journal={Nature structural & molecular biology},
  pages={430 - 437}
  • M. A. Bayfield, R. Maraia
  • Published 2009
  • Biology, Medicine
  • Nature structural & molecular biology
  • La proteins bind pre-tRNAs at their UUU-3′OH ends, facilitating their maturation. Although the mechanism by which La binds pre-tRNA 3′ trailers is known, the function of the RNA binding β-sheet surface of the RNA-recognition motif (RRM1) is unknown. How La dissociates from UUU-3′OH–containing trailers after 3′ processing is also unknown. Here we show that La preferentially binds pre-tRNAs over processed tRNAs or 3′ trailer products through coupled use of two sites: one on the La motif and… CONTINUE READING
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