• Chemistry, Medicine
  • Published in
    The Journal of biological…
    1978

Precursor of egg white ovomucoid. Amino acid sequence of an NH2-terminal extension.

@article{Thibodeau1978PrecursorOE,
  title={Precursor of egg white ovomucoid. Amino acid sequence of an NH2-terminal extension.},
  author={Stephen N. Thibodeau and Richard D Palmiter and Kenneth A. Walsh},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 24},
  pages={
          9018-23
        }
}
The translation of ovomucoid mRNA in a reticulocyte lysate protein-synthesizing system yields a precursor form which contains an NH2-terminal extension of 23 amino acid residues. Edman degradation of radioactive translation products (pre-ovomucoid) identified the following sequence: formula : (see text), where the initiator methionine (in parentheses) is the only residue cleaved from the NH2 terminus during cell-free synthesis and the vertical line indicates the site at which pre-ovomucoid is… CONTINUE READING