Precursor in cotranslational secretion of diphtheria toxin.

  title={Precursor in cotranslational secretion of diphtheria toxin.},
  author={W P Smith and Phang C. Tai and John A Murphy and Bernard D. Davis},
  journal={Journal of bacteriology},
  volume={141 1},
By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on these polysomes were completed in vitro, in the absence of membrane they were found to include not only diphtheria toxin of a molecular weight of 62,000, but also a larger… CONTINUE READING