Preclinical validation of fluorescence in situ hybridization assays for clinical practice

@article{Wiktor2006PreclinicalVO,
  title={Preclinical validation of fluorescence in situ hybridization assays for clinical practice},
  author={Anne E. Wiktor and Daniel L. Van Dyke and Peggy J Stupca and Rhett P. Ketterling and Erik C. Thorland and Brandon M Shearer and Stephanie R. Fink and Kimberly J Stockero and Jason R Majorowicz and Gordon W. Dewald},
  journal={Genetics in Medicine},
  year={2006},
  volume={8},
  pages={16-23}
}
Purpose: Validation of fluorescence in situ hybridization assays is required before using them in clinical practice. Yet, there are few published examples that describe the validation process, leading to inconsistent and sometimes inadequate validation practices. The purpose of this article is to describe a broadly applicable preclinical validation process.Methods: Validation is performed using four consecutive experiments. The Familiarization experiment tests probe performance on metaphase… CONTINUE READING