Preclinical evaluation of biotin labeling for red cell survival testing

  title={Preclinical evaluation of biotin labeling for red cell survival testing},
  author={Gertrud Hoffmann-Fezer and C. Trastl and Wolfgang Beisker and Duglas Berg and Josef Obermaier and Wolfram Kessler and Josef Mysliwietz and Michael Schumm and Juliane Filser and Stefan Thierfelder},
  journal={Annals of Hematology},
 Biotin labeling of red cells was tested in dogs as a preclinical study for cell survival. Red cells were labeled with either spacered Biotin-X-NHS (BxNHS) or water-soluble biotin compounds. After reinfusion, biotinylated red cells were detected in small blood samples (5 μl) with flow cytometry. Improved BxNHS labeling allows an easy detection of positive red cells for almost 100 days, whereas labeling with watersoluble compounds – despite strong labeling during the first days – results in a… CONTINUE READING
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