Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

@article{Maecker2007PrecisionAL,
  title={Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides},
  author={Holden T. Maecker and Jeffrey Hassler and Janice K. Payne and Amanda Summers and Karrie E Comatas and Manar Ghanayem and Michael Andew Morse and Timothy M. Clay and H Kim Lyerly and Sonny Bhatia and Smita A. Ghanekar and Vernon C. Maino and Corazon delaRosa and Mary Lenora Disis},
  journal={BMC Immunology},
  year={2007},
  volume={9},
  pages={9 - 9}
}
Single-cell assays of immune function are increasingly used to monitor T cell responses in immunotherapy clinical trials. Standardization and validation of such assays are therefore important to interpretation of the clinical trial data. Here we assess the levels of intra-assay, inter-assay, and inter-operator precision, as well as linearity, of CD8+ T cell IFNγ-based ELISPOT and cytokine flow cytometry (CFC), as well as tetramer assays. Precision was measured in cryopreserved PBMC with a low… CONTINUE READING

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