Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice.

  title={Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice.},
  author={Masafumi Mikami and Seiichi Toki and Masaki Endo},
  journal={Plant & cell physiology},
  volume={57 5},
Recent reports of CRISPR- (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) mediated heritable mutagenesis in plants highlight the need for accuracy of the mutagenesis directed by this system. Off-target mutations are an important issue when considering functional gene analysis, as well as the molecular breeding of crop plants with large genome size, i.e. with many duplicated genes, and where the whole-genome sequence is still lacking. In mammals… CONTINUE READING
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