Precise particle tracking against a complicated background: polynomial fitting with Gaussian weight.

@article{Rogers2007PrecisePT,
  title={Precise particle tracking against a complicated background: polynomial fitting with Gaussian weight.},
  author={Salman S. Rogers and Thomas Andrew Waigh and Xiubo Zhao and Jian Ren Lu},
  journal={Physical biology},
  year={2007},
  volume={4 3},
  pages={220-7}
}
We present a new particle tracking software algorithm designed to accurately track the motion of low-contrast particles against a background with large variations in light levels. The method is based on a polynomial fit of the intensity around each feature point, weighted by a Gaussian function of the distance from the centre, and is especially suitable for tracking endogeneous particles in the cell, imaged with bright field, phase contrast or fluorescence optical microscopy. Furthermore, the… CONTINUE READING
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