Precise maps of RNA polymerase reveal how promoters direct initiation and pausing.

@article{Kwak2013PreciseMO,
  title={Precise maps of RNA polymerase reveal how promoters direct initiation and pausing.},
  author={Hojoong Kwak and Nicholas J. Fuda and Leighton J. Core and John T Lis},
  journal={Science},
  year={2013},
  volume={339 6122},
  pages={950-3}
}
Transcription regulation occurs frequently through promoter-associated pausing of RNA polymerase II (Pol II). We developed a precision nuclear run-on and sequencing (PRO-seq) assay to map the genome-wide distribution of transcriptionally engaged Pol II at base pair resolution. Pol II accumulates immediately downstream of promoters, at intron-exon junctions that are efficiently used for splicing, and over 3' polyadenylation sites. Focused analyses of promoters reveal that pausing is not fixed… CONTINUE READING
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