Precise genome modification in the crop species Zea mays using zinc-finger nucleases

  title={Precise genome modification in the crop species Zea mays using zinc-finger nucleases},
  author={V. K. Shukla and Yannick Doyon and J. C. Miller and Russell Dekelver and E. Moehle and Sarah E. Worden and J. C. Mitchell and Nicole L. Arnold and Sunita M. Gopalan and Xiangdong Meng and Vivian M. Choi and J. Rock and Ying-ying Wu and George E. Katibah and G. Zhi-fang and David G. Mccaskill and M. Simpson and B. Blakeslee and Scott A. Greenwalt and H. Butler and Sarah J. Hinkley and L. Zhang and E. Rebar and P. Gregory and F. Urnov},
  • V. K. Shukla, Yannick Doyon, +22 authors F. Urnov
  • Published 2009
  • Biology, Medicine
  • Nature
  • Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify… CONTINUE READING
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