Precise genome modification in the crop species Zea mays using zinc-finger nucleases

@article{Shukla2009PreciseGM,
  title={Precise genome modification in the crop species Zea mays using zinc-finger nucleases},
  author={Vipula K. Shukla and Yannick Doyon and Jeffrey C. Miller and Russell Dekelver and Erica A. Moehle and Sarah E. Worden and Jon C. Mitchell and Nicole L. Arnold and Sunita M. Gopalan and Xiangdong Meng and Vivian M. Choi and Jeremy M. Rock and Ying-Ying Wu and George E. Katibah and Gao Zhi-fang and David G. McCaskill and Matthew A. Simpson and Beth Blakeslee and Scott A. Greenwalt and Holly Butler and Sarah Hinkley and Lei Zhang and Edward J Rebar and Philip D. Gregory and Fyodor D. Urnov},
  journal={Nature},
  year={2009},
  volume={459},
  pages={437-441}
}
Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify… 
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