Precise excision of Tn5 in Escherichia coli K12 mutants with alterations in common component of phosphotransferase system and in DNA-gyrase subunits


The effect of ptsH, gyrA and gyrB mutations on the frequency of precise excision (PE) of transposon Tn5 was studied in Escherichia coli K12. The conjugative plasmid with Tn5 integrated in the tet gene of Tn10 was used as a model in the experiments. It was shown that mutational damage to the HPr protein (ptsH mutation), a common component of the bacterial… (More)
DOI: 10.3103/S0891416810010039


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