Precise excision of TTAA‐specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera

@article{Fraser1996PreciseEO,
  title={Precise excision of TTAA‐specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera},
  author={Malcolm J. Fraser and T. Clszczon and Teresa A. Elick and Christopher A. Bauser},
  journal={Insect Molecular Biology},
  year={1996},
  volume={5}
}
Transposon mutagenesis of baculoviruses provides an ideal experimental system for analysis of the movement of a unique family of mobile element identified from lepidopteran genomes. Members of this family of short‐inverted‐repeat elements are characterized by their extreme Specificity for TTAA target sites. This report describes the analysis of excision events for two representatives of this family, tagalong (formerly TFP3) and piggysac (formerly IFP2). These elements were tagged with a… 
piggyBac Transposon-Mediated Transgenesis in the Apicomplexan Parasite Eimeria tenella
TLDR
Southern-blotting analysis showed that exogenous DNA containing the EYFP gene was integrated into the parasite genome at a limited number of integration sites and that the inserted part of the donor plasmid was the fragment located between the 5′ and 3′ ITRs as indicated by primer-specific PCR screening.
The piggyBac Transposon Displays Local and Distant Reintegration Preferences and Can Cause Mutations at Noncanonical Integration Sites
TLDR
It is found that piggyBac preferentially integrates locally to the excision site when mobilized from a chromosomal location and identified other nonlocal regions of the genome with elevated insertion frequencies.
Evolution of the Xenopus piggyBac transposon family TxpB: domesticated and untamed strategies of transposon subfamilies.
TLDR
The present results support the viewpoint that some Uribo2 members are naturally active autonomous transposons, whereas Kobuta members may be domesticated by hosts.
Formation and loss of large, unstable tandem arrays of the piggyBac transposable element in the yellow fever mosquito, Aedes aegypti
TLDR
The results reveal one possible outcome of uncontrolled and/or unexpected recombination in this mosquito, and support the conclusion that further investigation is necessary before transposable elements such as piggyBac can be used as genetic drive mechanisms to move pathogen-resistance genes into mosquito populations.
Excision of the piggyBac transposable element in vitro is a precise event that is enhanced by the expression of its encoded transposase
TLDR
The results suggest that the piggyBac transposon encodes a protein that functions to facilitate not only insertion, but precise excision as well, which is the first demonstration of pigGYBac mobility from plasmid sources in uninfected Lepidopteran cells.
A non-autonomous insect piggyBac transposable element is mobile in tobacco
TLDR
Data indicate that piggyBac transposase is active in a dicotyledonous plant, although at a low frequency, according to a two-element system developed in tobacco.
A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe
TLDR
It is shown that PB transposes in the fission yeast Schizosaccharomyces pombe and leaves almost no footprints, and PB transposition in this strain has low chromosomal distribution bias as shown by deep sequencing-based insertion site mapping.
Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap‐3) by a new class‐II piggyBac‐related insect transposon
A new piggyBac–related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus
Chimeric Mos1 and piggyBac transposases result in site‐directed integration
TLDR
A novel technology that utilizes chimeric transposases to direct integration into specific sites on a target DNA molecule has the potential to minimize nonspecific integration events that may result in insertional mutagenesis and reduced fitness.
Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences
TLDR
This survey allows the first comparative examination of the distinctive piggyBac transposase, suggesting that it might contain a highly divergent DDD domain, comparable to the widespread DDE domain found in many DNA transposases and retroviral integrases.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 50 REFERENCES
Transposon mutagenesis of baculoviruses: analysis of TFP3 lepidopteran transposon insertions at the FP locus of nuclear polyhedrosis viruses.
TLDR
Sequence analysis at the excision site of a spontaneous revertant demonstrates that the TFP3 elements are capable of precise excision, restoring the expression of the 25-kDa protein.
Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA.
TLDR
This assay provides the first demonstration that a Lepidopteran transposon is capable of transposing while carrying a marker gene in insect cells, and provides strong evidence that IFP2 encodes a protein that facilitates its own movement.
TTAA serves as the target site for TFP3 lepidopteran transposon insertions in both nuclear polyhedrosis virus and Trichoplusia ni genomes
TLDR
TFP3 transposable elements from five independently isolated FP mutants of the Autographa californica nuclear polyhedrosis virus suggest that mobilization of TFP3 into both viral and cellular sites is identical in specificity and mechanism.
Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses.
TLDR
The transposable IFP2 element of Trichoplusia ni was originally isolated as a host DNA insertion in spontaneous FP mutants of Galleria mellonella or Autographa californica nuclear polyhedrosis viruses, but is not apparent in DNAs isolated from the TN-R2 cell line or the authors' laboratory colony of T. ni larvae, suggesting IFP1 was recently introduced into the T. Ni genome.
The Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-type element of the hobo, Ac, and Tam3 (hAT) element family.
TLDR
Comparison of the ends of the Hermes and hobo elements to those of the Ac element of Zea mays, and the Tam3 element of Antirrhinum majus, as well as several other plant and insect elements, revealed a conserved terminal sequence motif and Hermes is clearly a member of the hobo, Ac and Tam3 transposable element family.
The hobo transposable element of Drosophila can be cross-mobilized in houseflies and excises like the Ac element of maize.
TLDR
The hobo transposable element from Drosophila melanogaster was found to be capable of excision, resulting in donor sites unlike those reported for any other transposability element currently known in animals, and appears to be a mobile-element system related to hobo, Ac, and Tam3.
Acquisition of Host Cell DNA Sequences by Baculoviruses: Relationship Between Host DNA Insertions and FP Mutants of Autographa californica and Galleria mellonella Nuclear Polyhedrosis Viruses
TLDR
Mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses, which produce an altered plaque phenotype as a result of reduced numbers of viral occlusions in infected cells, were isolated after passage in Trichoplusia ni (TN-368) cells and it was suggested that the insertion of cell DNA into the viral genomes resulted in the FP plaque phenotype.
Sequence comparison of cellular and viral copies of host cell DNA insertions found in Autographa californica nuclear polyhedrosis virus.
The nucleotide sequence and structural characteristics of two nonhomologous host DNA insertions of Spodoptera frugiperda (fall armyworm) origin isolated from few polyhedra mutants of the baculovirus
Comparisons of host cell DNA insertions and altered transcription at the site of insertions in few polyhedra bacilovirus mutants.
TLDR
Cell-free translation of cRNAs transcribed from wild-type viral DNA revealed an open reading frame coding for a 25-kDa protein at the site where host cell DNA insertions have been mapped, the same size as an infected-cell protein missing from most FP mutants examined.
Location and nucleotide sequence of the 25K protein missing from baculovirus few polyhedra (FP) mutants.
Wild-type and few polyhedra (FP) mutants of the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) were studied to identify and sequence the gene encoding the 25-kDa (25K) protein
...
1
2
3
4
5
...