Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes.

@article{Leiding2009PreciseDO,
  title={Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes.},
  author={Thom Leiding and K. G{\'o}recki and T. Kjellman and S. Vinogradov and C. H{\"a}gerh{\"a}ll and S. P. {\AA}rsk{\"o}ld},
  journal={Analytical biochemistry},
  year={2009},
  volume={388 2},
  pages={
          296-305
        }
}
Accurate real-time measurements of proton concentration gradients are pivotal to mechanistic studies of proton translocation by membrane-bound enzymes. Here we report a detailed characterization of the pH-sensitive fluorescent nanoprobe Glu(3), which is well suited for pH measurements in microcompartmentalized biological systems. The probe is a polyglutamic porphyrin dendrimer in which multiple carboxylate termini ensure its high water solubility and prevent its diffusion across phospholipid… Expand
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