Precautions when determining kinetically the order of inactivation of enzymes by functionally irreversible inhibitors.

@article{Carlson1984PrecautionsWD,
  title={Precautions when determining kinetically the order of inactivation of enzymes by functionally irreversible inhibitors.},
  author={G. M. Carlson},
  journal={Biochimica et biophysica acta},
  year={1984},
  volume={789 3},
  pages={
          347-50
        }
}
The number of molecules of an irreversible inhibitor that are responsible for inactivation of a catalytic site is often deduced from the slope of a plot of the log of the apparent rate of inactivation (k') at different concentrations of inhibitor versus the log of the inhibitor concentrations. The purpose of this note is to urge caution in experimental design and interpretation if one attempts to utilize this kinetic technique to characterize the order of inactivation brought about by… CONTINUE READING