Preactivation permits subsequent stimulation of phospholipase C by G(i)-coupled receptors.

@article{Chan2000PreactivationPS,
  title={Preactivation permits subsequent stimulation of phospholipase C by G(i)-coupled receptors.},
  author={J S Chan and J W Lee and Maurice K. C. Ho and Yung Ho Kenny Wong},
  journal={Molecular pharmacology},
  year={2000},
  volume={57 4},
  pages={700-8}
}
In the complex signal transduction networks involving G protein-coupled receptors there are numerous examples where G(i)-linked receptors augment G(q)-dependent signals. The mechanistic basis of such occurrences is thought to entail signal convergence at phospholipase Cbeta (PLCbeta) via the G protein betagamma-dimers. Herein, we explored the possibility that augmentation by betagamma-dimers requires preactivation of PLCbeta. COS-7 cells were transiently cotransfected with cDNAs encoding… CONTINUE READING