Pre-treatment of Caco-2 cells with zinc during the differentiation phase alters the kinetics of zinc uptake and transport(2).

@article{Reeves2001PretreatmentOC,
  title={Pre-treatment of Caco-2 cells with zinc during the differentiation phase alters the kinetics of zinc uptake and transport(2).},
  author={Philip Reeves and Mary Briske-Anderson and LuAnn K Johnson},
  journal={The Journal of nutritional biochemistry},
  year={2001},
  volume={12 12},
  pages={674-684}
}
The Caco-2 cell model was used to study the efficiency of absorption and endogenous excretion of zinc (Zn) regulated by dietary Zn concentration. Cells were seeded onto high pore-density membranes and maintained in medium supplemented with 10% FBS. After confluence, cells were treated with 5 or 25 &mgr;mol Zn/L for 7 d, and Zn uptake and transport were measured in both apical (AP) and basolateral (BL) directions by using (65)Zn. Similar cells were labeled with (65)Zn and the release of Zn to… CONTINUE READING

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