Pre-mRNA splicing mutants of Schizosaccharomyces pombe.

  title={Pre-mRNA splicing mutants of Schizosaccharomyces pombe.},
  author={Judith A Potashkin and Rong Li and D. A. Frendewey},
  journal={The EMBO journal},
  volume={8 2},
A collection of temperature sensitive (ts-) mutants was prepared by chemical mutagenesis of a wild type Schizosaccharomyces pombe strain. To screen the ts- mutants for pre-mRNA splicing defects, an oligodeoxynucleotide that recognizes one of the introns of the beta-tubulin pre-mRNA was used as a probe in a Northern blot assay to detect accumulation of intron sequences. This screening procedure identified three pre-mRNA splicing mutants from 100 ts- strains. The three mutants are defective in an… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 25 extracted citations

Similar Papers

Loading similar papers…