Pre-mRNA splicing mutants of Schizosaccharomyces pombe.

@article{Potashkin1989PremRNASM,
  title={Pre-mRNA splicing mutants of Schizosaccharomyces pombe.},
  author={Judith A Potashkin and Rong Li and D. A. Frendewey},
  journal={The EMBO journal},
  year={1989},
  volume={8 2},
  pages={551-9}
}
A collection of temperature sensitive (ts-) mutants was prepared by chemical mutagenesis of a wild type Schizosaccharomyces pombe strain. To screen the ts- mutants for pre-mRNA splicing defects, an oligodeoxynucleotide that recognizes one of the introns of the beta-tubulin pre-mRNA was used as a probe in a Northern blot assay to detect accumulation of intron sequences. This screening procedure identified three pre-mRNA splicing mutants from 100 ts- strains. The three mutants are defective in an… CONTINUE READING

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