Pre-PCR processing: strategies to generate PCR-compatible samples.

  title={Pre-PCR processing: strategies to generate PCR-compatible samples.},
  author={Peter R{\aa}dstr{\"o}m and Rickard Knutsson and Petra T. Wolffs and Maria L{\"o}venklev and Charlotta L{\"o}fstr{\"o}m},
  journal={Molecular biotechnology},
  volume={26 2},
Polymerase chain reaction (PCR) is recognized as a rapid, sensitive, and specific molecular diagnostic tool for the analysis of nucleic acids. However, the sensitivity and kinetics of diagnostic PCR may be dramatically reduced when applied directly to biological samples, such as blood and feces, owing to PCR-inhibitory components. As a result, pre-PCR processing procedures have been developed to remove or reduce the effects of PCR inhibitors. Pre-PCR processing comprises all steps prior to the… CONTINUE READING

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