Pre-B Cell Receptor Signaling Induces Immunoglobulin κ Locus Accessibility by Functional Redistribution of Enhancer-Mediated Chromatin Interactions

@inproceedings{Stadhouders2014PreBCR,
  title={Pre-B Cell Receptor Signaling Induces Immunoglobulin κ Locus Accessibility by Functional Redistribution of Enhancer-Mediated Chromatin Interactions},
  author={Ralph Stadhouders and Marjolein J. W. de Bruijn and Magdalena B. Rother and Saravanan Yuvaraj and Claudia Ribeiro de Almeida and Petros Kolovos and Menno C van Zelm and Wilfred F J van Ijcken and Frank Grosveld and Eric Soler and Rudi W Hendriks},
  booktitle={PLoS biology},
  year={2014}
}
During B cell development, the precursor B cell receptor (pre-BCR) checkpoint is thought to increase immunoglobulin κ light chain (Igκ) locus accessibility to the V(D)J recombinase. Accordingly, pre-B cells lacking the pre-BCR signaling molecules Btk or Slp65 showed reduced germline V(κ) transcription. To investigate whether pre-BCR signaling modulates V(κ) accessibility through enhancer-mediated Igκ locus topology, we performed chromosome conformation capture and sequencing analyses. These… CONTINUE READING