Pre‐mRNA processing enhancer (PPE) element increases the expression of an intronless thymidylate synthase gene but does not affect intron‐dependent S phase regulation

@article{Lee1998PremRNAPE,
  title={Pre‐mRNA processing enhancer (PPE) element increases the expression of an intronless thymidylate synthase gene but does not affect intron‐dependent S phase regulation},
  author={Taohua Lee and Lee F. Johnson},
  journal={Journal of Cellular Biochemistry},
  year={1998},
  volume={69}
}
  • T. Lee, L. Johnson
  • Published 1998
  • Biology, Medicine
  • Journal of Cellular Biochemistry
The pre‐mRNA processing enhancer (PPE) element is an RNA sequence element derived from the intronless HSV‐TK gene. Insertion of the element into the highly intron‐dependent human β‐globin gene leads to efficient expression in the absence of splicing. We have analyzed the effect of the PPE element on the expression of mouse thymidylate synthase (TS) minigenes. We have previously shown that the expression of intronless TS minigenes is moderately (up to 20‐fold) stimulated by the inclusion of… Expand
Transcriptional control elements and complex initiation pattern of the TATA‐less bidirectional human thymidylate synthase promoter
TLDR
The nucleotide sequences that are important for transcription of the human thymidylate synthase gene were analyzed by deletion and site‐directed mutagenesis of the promoter region and this region, which is highly conserved in human, mouse and rat TS promoters, was designated the essential promoter region. Expand
Inactivation of MED‐1 elements in the TATA‐less, initiator‐less mouse thymidylate synthase promoter has no effect on promoter strength or the complex pattern of transcriptional start sites
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It is concluded that the MED‐1 element does not play a significant role in TS promoter function and therefore is not an essential component of all TATA‐less promoters with complex transcriptional initiation patterns. Expand
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Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer.
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