Pre‐mRNA processing enhancer (PPE) element increases the expression of an intronless thymidylate synthase gene but does not affect intron‐dependent S phase regulation

  title={Pre‐mRNA processing enhancer (PPE) element increases the expression of an intronless thymidylate synthase gene but does not affect intron‐dependent S phase regulation},
  author={Taohua Lee and Lee F. Johnson},
  journal={Journal of Cellular Biochemistry},
  • T. Lee, L. Johnson
  • Published 1998
  • Biology, Medicine
  • Journal of Cellular Biochemistry
The pre‐mRNA processing enhancer (PPE) element is an RNA sequence element derived from the intronless HSV‐TK gene. Insertion of the element into the highly intron‐dependent human β‐globin gene leads to efficient expression in the absence of splicing. We have analyzed the effect of the PPE element on the expression of mouse thymidylate synthase (TS) minigenes. We have previously shown that the expression of intronless TS minigenes is moderately (up to 20‐fold) stimulated by the inclusion of… Expand
Transcriptional control elements and complex initiation pattern of the TATA‐less bidirectional human thymidylate synthase promoter
The nucleotide sequences that are important for transcription of the human thymidylate synthase gene were analyzed by deletion and site‐directed mutagenesis of the promoter region and this region, which is highly conserved in human, mouse and rat TS promoters, was designated the essential promoter region. Expand
Inactivation of MED‐1 elements in the TATA‐less, initiator‐less mouse thymidylate synthase promoter has no effect on promoter strength or the complex pattern of transcriptional start sites
It is concluded that the MED‐1 element does not play a significant role in TS promoter function and therefore is not an essential component of all TATA‐less promoters with complex transcriptional initiation patterns. Expand
Arginine-Rich Regions Mediate the RNA Binding and Regulatory Activities of the Protein Encoded by the Drosophila melanogaster suppressor of sable Gene
It is proposed that SU(S) binds to the 5′ region of nascent transcripts and inhibits RNA production in a manner that can be overcome by splicing complex assembly. Expand
Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer.
This preliminary study suggests that TS repetitive-sequence polymorphisms are predictive for tumor downstaging and may be useful as a novel means of predicting response to preoperative 5-FU-based chemoradiation. Expand


HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
It is concluded that hnRNP L binds in a sequence-specific manner to this RNA sequence element that enables intron-independent gene expression, and that introns-independent pre-mRNA processing and transport involves sequence- specific RNA-protein interactions between cis-acting RNA sequence elements and proteins such as hn RNP L. Expand
Regulation of mouse thymidylate synthase gene expression in growth-stimulated cells: upstream S phase control elements are indistinguishable from the essential promoter elements.
It appears that the mammalian thymidylate synthase gene is controlled primarily at the posttranscriptional level, and that the TS essential promoter region is necessary (although not sufficient) for proper S phase regulation. Expand
The mouse histone H2a gene contains a small element that facilitates cytoplasmic accumulation of intronless gene transcripts and of unspliced HIV-1-related mRNAs.
  • Y. Huang, G. Carmichael
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1997
It is demonstrated here that a 100-bp sequence within the H2a coding region permits efficient cytoplasmic accumulation of the globin cDNA transcripts and shown that this sequence appears to suppress splicing and can functionally replace Rev and the Rev-responsive element in the cy toplasmo accumulation of unspliced HIV-1-related mRNAs. Expand
The 5'-flanking region of the mouse thymidylate synthase gene is necessary but not sufficient for normal regulation in growth-stimulated cells.
Observations indicate that sequences upstream of the transcriptional start sites of the TS gene are necessary, although not sufficient, for normal growth-regulated expression of the mouse TS gene. Expand
Splicing signals are required for S-phase regulation of the mouse thymidylate synthase gene
It is shown that both the promoter of the mouse TS gene and TS introns are necessary (although neither is sufficient) for S-phase-specific regulation of TS mRNA content, and that the splicing reaction itself, rather than a control sequence within the intron, is important for growth-regulated expression of the TS gene. Expand
Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence.
Observations support the idea that introns can stimulate gene expression by a process that depends directly on the splicing reaction. Expand
Introns are essential for growth-regulated expression of the mouse thymidylate synthase gene.
Observations indicate that TS introns contain sequences that are necessary for normal growth-regulated expression of the mouse TS gene. Expand
Comparison of intron-dependent and intron-independent gene expression.
Sequences from the transcribed region of the herpes simplex virus thymidine kinase gene, a gene that lacks an intervening sequence, permitted substantial intron-independent expression (greater than 100-fold increase) in the plasmid vector system. Expand
Thymidylate synthase gene expression is stimulated by some (but not all) introns.
Analysis of minigenes that contained various combinations of introns revealed that the stimulatory effects of the introns were not additive, and almost all of intron 5 and 6 could be deleted without diminishing the stimulatories effect. Expand
Lack of an initiator element is responsible for multiple transcriptional initiation sites of the TATA-less mouse thymidylate synthase promoter.
The results indicate that there are no additional promoter elements downstream of the G/C box in the initiation window of mouse thymidylate synthase and why transcription initiates across such a broad region is determined. Expand