Practolol metabolism. III. Irreversible binding of [14C]practolol metabolite(s) to mammalian liver microsomes.

@article{Orton1981PractololMI,
  title={Practolol metabolism. III. Irreversible binding of [14C]practolol metabolite(s) to mammalian liver microsomes.},
  author={Terry C. Orton and Clinton Lowery},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1981},
  volume={219 1},
  pages={
          207-12
        }
}
The irreversible binding of [14C]practolol metabolites to mammalian liver microsomes was studied in vitro. Binding occurred by covalent linkage to amino acids of microsomal proteins. The binding reaction was dependent on oxygen, NADPH, reaction time, microsomal protein content and substrate concentration and was inhibited by carbon monoxide, glutathione and albumin. Albumin inhibition resulted in the binding of reactive metabolite(s) to this serum protein. Hamster (Syrian) liver microsomes had… CONTINUE READING