Practical labeling methodology for choline-derived lipids and applications in live cell fluorescence imaging.

@article{Li2014PracticalLM,
  title={Practical labeling methodology for choline-derived lipids and applications in live cell fluorescence imaging.},
  author={Caishun Li and Jessie A. Key and Feng Jia and Arpan Dandapat and Soo Kyun Hur and Christopher W. Cairo},
  journal={Photochemistry and photobiology},
  year={2014},
  volume={90 3},
  pages={
          686-95
        }
}
Lipids of the plasma membrane participate in a variety of biological processes, and methods to probe their function and cellular location are essential to understanding biochemical mechanisms. Previous reports have established that phosphocholine-containing lipids can be labeled by alkyne groups through metabolic incorporation. Herein, we have tested alkyne, azide and ketone-containing derivatives of choline as metabolic labels of choline-containing lipids in cells. We also show that 17… CONTINUE READING
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