Practical issues in the application of oxygenases.

@article{Beilen2003PracticalII,
  title={Practical issues in the application of oxygenases.},
  author={Jan B van Beilen and Wouter A. Duetz and Andreas Schmid and Bernard Witholt},
  journal={Trends in biotechnology},
  year={2003},
  volume={21 4},
  pages={170-7}
}
Oxygenases carry out the regio-, stereo- and chemoselective introduction of oxygen in a tremendous range of organic molecules. This versatility has already been exploited in several commercial processes. There are, however, many hurdles to further practical large-scale applications. Here, we review various issues in biocatalysis using these enzymes, such as screening strategies, overoxidation, uncoupling, substrate uptake, substrate toxicity, and oxygen mass transfer. By addressing these issues… CONTINUE READING

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