Practical determination of hyaluronan by a new noncompetitive fluorescence-based assay on serum of normal and cirrhotic patients.

@article{Martins2003PracticalDO,
  title={Practical determination of hyaluronan by a new noncompetitive fluorescence-based assay on serum of normal and cirrhotic patients.},
  author={Jo{\~a}o Roberto Maciel Martins and Carlo Camargo Passerotti and Rui Monteiro de Barros Maciel and Lucia O. Sampaio and Carl Dietrich and Helena B. Nader},
  journal={Analytical biochemistry},
  year={2003},
  volume={319 1},
  pages={65-72}
}
A practical fluorescence-based assay method for determination of hyaluronan (HA) was developed. Plates were coated with hyaluronan-binding proteins (HABP) obtained from bovine cartilage and successively incubated with samples containing standard solutions of hyaluronan or serum from normal and cyrrhotic patients, biotin-conjugated HABP, and europium-labeled streptavidin. After release of europium from streptavidin with enhancement solution the final fluorescence is measured in a fluorometer… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 29 extracted citations

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas • 2015
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…