Practical aspects of NMR-based fragment screening.

  title={Practical aspects of NMR-based fragment screening.},
  author={Christopher A Lepre},
  journal={Methods in enzymology},
NMR spectroscopy is a popular and highly versatile screening method for fragment-based drug discovery. NMR methods are capable of robustly detecting the binding of fragments to macromolecular targets over an extraordinarily broad affinity range (from covalent to millimolar). This chapter provides a stepwise process for creating an NMR-based fragment screening program. The construction of fragment libraries is described, including compound selection, plating of stocks, and preparation of… CONTINUE READING

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