Practical Considerations for Using Pooled Lentiviral CRISPR Libraries

  title={Practical Considerations for Using Pooled Lentiviral CRISPR Libraries},
  author={Joel Ryan McDade and Nicole C. Waxmonsky and Lianna E. Swanson and Melina Fan},
  journal={Current Protocols in Molecular Biology},
  pages={31.5.1 - 31.5.13}
CRISPR/Cas9 technology is ideally suited for genome‐wide screening applications due to the ease of generating guide RNAs (gRNAs) and the versatility of Cas9 or Cas9 derivatives to knockout, repress, or activate expression of target genes. Several pooled lentiviral CRISPR libraries have been developed and are now publicly available, but while using CRISPR/Cas9 for genetic experiments has become widely adopted, genome‐wide screening experiments remain technically challenging. This review covers… 

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