Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction.

@article{Dionisi2003PowerAF,
  title={Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction.},
  author={Hebe M. Dionisi and Gerda Harms and Alice C. Layton and Igrid R Gregory and Jack Parker and Shawn A. Hawkins and Kevin G. Robinson and Gary S. Sayler},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 11},
  pages={
          6597-604
        }
}
The aims of this study were to determine the power of discrimination of the real-time PCR assay for monitoring fluctuations in microbial populations within activated sludge and to identify sample processing points where methodological changes are needed to minimize the variability in target quantification. DNA was extracted using a commercially available kit from mixed liquor samples taken from the aeration tank of four bench-scale activated-sludge reactors operating at 2-, 5-, 10-, and 20-day… CONTINUE READING

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