Potentiometric analysis of the flavin cofactors of neuronal nitric oxide synthase.

  title={Potentiometric analysis of the flavin cofactors of neuronal nitric oxide synthase.},
  author={Michael A Noble and Andrew W Munro and Stuart L. Rivers and Liliana Anguiano Robledo and Simon N Daff and Lesley J. Yellowlees and Toru Shimizu and Ikuko Sagami and J Guy Guillemette and Stephen K. Chapman},
  volume={38 50},
Midpoint reduction potentials for the flavin cofactors in the reductase domain of rat neuronal nitric oxide synthase (nNOS) in calmodulin (CaM)-free and -bound forms have been determined by direct anaerobic titration. In the CaM-free form, the FMN potentials are -49 +/- 5 mV (oxidized/semiquinone) -274 +/- 5 mV (semiquinone/reduced). The corresponding FAD potentials are -232 +/- 7, and -280 +/- 6 mV. The data indicate that each flavin can exist as a blue (neutral) semiquinone. The accumulation… CONTINUE READING
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