Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b.


Antibody-dependent cytolytic effector lymphocytes are known to possess, in part, receptors for activated C3. Employing a model system consisting of 51Cr-labeled chicken erythrocytes and purified human peripheral lymphocytes, we investigated the effect of target cell bound C3b on antibody-dependent cellular cytotoxicity (ADCC). At concentrations of anti-target cell antibody too low to cause effective ADCC, target cell bound C3b cooperated with antibody to produce marked target cell lysis. In the presence of a 1/6.25 X 10(6) dilution of anti-chicken erythrocyte rabbit IgG, cell lysis increased from 20% to 65% by the attachment of 18,000 C3b molecules per cell. C3b-dependent enhancement of ADCC was dose dependent. It was augmented by attachment of activated properdin (P) to the C3b-bearing target cells.


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@article{Ghebrehiwet1979PotentiationOA, title={Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b.}, author={Berhane Ghebrehiwet and R G Medicus and Hans J. M{\"{u}ller-Eberhard}, journal={Journal of immunology}, year={1979}, volume={123 3}, pages={1285-8} }