Potentiation of GPCR-signaling via membrane targeting of G protein alpha subunits.

@article{Kostenis2002PotentiationOG,
  title={Potentiation of GPCR-signaling via membrane targeting of G protein alpha subunits.},
  author={Evi Kostenis},
  journal={Journal of receptor and signal transduction research},
  year={2002},
  volume={22 1-4},
  pages={267-81}
}
  • Evi Kostenis
  • Published 2002 in
    Journal of receptor and signal transduction…
Different assay technologies are available that allow ligand occupancy of G protein coupled receptors to be converted into robust functional assay signals. Of particular interest are universal screening systems such that activation of any GPCR can be detected with a common assay end point. The promiscuous G protein Galpha16 and chimeric G proteins are broadly used tools for setting up almost universal assay systems. Many efforts focused on making G proteins more promiscuous, however no attempts… CONTINUE READING