Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid).

  title={Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid).},
  author={Bryan D. Smith and Matthew B. Soellner and Ronald T. Raines},
  journal={The Journal of biological chemistry},
  volume={278 23},
Ribonuclease A (RNase A) can make multiple contacts with an RNA substrate. In particular, the enzymatic active site and adjacent subsites bind sequential phosphoryl groups in the RNA backbone through Coulombic interactions. Here, oligomers of vinylsulfonic acid (OVS) are shown to be potent inhibitors of RNase A that exploit these interactions. Inhibition is competitive with substrate and has Ki = 11 pm in assays at low salt concentration. The effect of salt concentration on inhibition indicates… CONTINUE READING
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Common Buffer Contaminant Inhibits Ribonuclease A

  • B. M. Fisher, J. E. Grilley, R. T. Raines
  • J. Biol. Chem. 273,
  • 1998

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