Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid).

@article{Smith2003PotentIO,
  title={Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid).},
  author={Bryan D. Smith and Matthew B. Soellner and Ronald T. Raines},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 23},
  pages={20934-8}
}
Ribonuclease A (RNase A) can make multiple contacts with an RNA substrate. In particular, the enzymatic active site and adjacent subsites bind sequential phosphoryl groups in the RNA backbone through Coulombic interactions. Here, oligomers of vinylsulfonic acid (OVS) are shown to be potent inhibitors of RNase A that exploit these interactions. Inhibition is competitive with substrate and has Ki = 11 pm in assays at low salt concentration. The effect of salt concentration on inhibition indicates… CONTINUE READING
27 Extracted Citations
1 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 27 extracted citations

Referenced Papers

Publications referenced by this paper.

Common Buffer Contaminant Inhibits Ribonuclease A

  • B. M. Fisher, J. E. Grilley, R. T. Raines
  • J. Biol. Chem. 273,
  • 1998

Similar Papers

Loading similar papers…