Corpus ID: 18821026

Potent bile salt and organic anion inhibition of methotrexate uptake and accumulation in the freshly isolated rat hepatocyte.

@article{Gewirtz1980PotentBS,
  title={Potent bile salt and organic anion inhibition of methotrexate uptake and accumulation in the freshly isolated rat hepatocyte.},
  author={David Gewirtz and Joyce K. Randolph and I. David Goldman},
  journal={Cancer research},
  year={1980},
  volume={40 6},
  pages={
          1852-7
        }
}
  • David Gewirtz, Joyce K. Randolph, I. David Goldman
  • Published in Cancer research 1980
  • Medicine
  • Abstract Influx of [3H]methotrexate into freshly isolated hepatocytes is mediated by high- and low-affinity processes, both of which are inhibited by the bile salts, cholate, taurocholate, and deoxycholate, and the organic anions, bromosulfophthalein and rose bengal. At 100 µm concentrations, these compounds inhibit 1 µm [3H]methotrexate influx by 70 to 90%. Dixon plots established a similar Ki (∼20 µm) for taurocholate inhibition of the high- and low-affinity influx routes for methotrexate… CONTINUE READING

    Citations

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    Characterization of the bile acid sensitive methotrexate carrier of rat liver cells

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    CITES BACKGROUND & RESULTS
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    Transport of Folates and Antifolates in Liver

    • Donald W. Horne
    • Biology, Medicine
    • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
    • 1993

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