Potent anti-tumor effects of an active site mutant of human manganese-superoxide dismutase. Evolutionary conservation of product inhibition.

@article{Davis2004PotentAE,
  title={Potent anti-tumor effects of an active site mutant of human manganese-superoxide dismutase. Evolutionary conservation of product inhibition.},
  author={Christopher A. Davis and Amy S Hearn and Bradley S. Fletcher and Justin S Bickford and Jorge Esparteiro Garcia and Vincent J-P L{\'e}v{\^e}que and Juan Andres Melendez and David N. Silverman and James R. Zucali and Anupam Agarwal and Harry S. Nick},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 13},
  pages={12769-76}
}
Mn-SOD serves as the primary cellular defense against oxidative damage by converting superoxide radicals (O(2)(-)) to O(2) and H(2)O(2). A unique characteristic of this mitochondrial anti-oxidant enzyme is the conservation from bacteria to man of a rapidly formed product inhibited state. Using site-directed mutagenesis, we have generated an active site mutant (H30N) of human Mn-SOD, which exhibits significantly reduced product inhibition and increased enzymatic efficiency. Overexpression of the… CONTINUE READING

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