Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

@article{Fire1998PotentAS,
  title={Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans},
  author={A. Fire and Siqun Xu and Mary Katherine Montgomery and Steven A. Kostas and Samuel E. Driver and Craig C Mello},
  journal={Nature},
  year={1998},
  volume={391},
  pages={806-811}
}
Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. [] Key Result After injection into adult animals, purified single strands had at most a modest effect, whereas double-stranded mixtures caused potent and specific interference. The effects of this interference were evident in both the injected animals and their progeny.
RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans.
TLDR
It is observed by in situ hybridization that dsRNA-mediated interference produced a substantial, although not complete, reduction in accumulation of nascent transcripts in the nucleus, while cytoplasmic accumulation of transcripts was virtually eliminated, indicating that the endogenous mRNA is the target for interference and suggesting a mechanism that degrades the targeted RNA before translation can occur.
Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans
TLDR
RNAi by feeding can provide significant information about the functions of an individual gene beyond that provided by injection and can be used for special applications for which injection or the use of mutants is sometimes impracticable.
RNA Interference in Biology and Medicine
First discovered in plants the nematode Caenorhabditis elegans, the production of small interfering RNAs (siRNAs) that bind to and induce the degradation of specific endogenous mRNAs is now
Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.
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It is shown that genetic interference by dsRNA can be achieved in a heritable and inducible fashion in Trypanosoma brucei and these new developments make it possible to initiate the molecular dissection of RNAi both biochemically and genetically.
The specifics of small interfering RNA specificity
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  • 2003
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Biochemical evidence indicates the siRNAs are incorporated into a multisubunit protein complex, the RNAi-induced silencing complex (RISC), which directs the siRNA to the appropriate mRNA.
RNA interference may result in unexpected phenotypes in Caenorhabditis elegans
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It is found that the reduced embryonic viability of mir-35-41(gk262) mutants is partially reversed by exogenous dsRNA via a mechanism that involves canonical RNAi, indicating cross-regulation between different small RNA pathways in C. elegans to regulate embryonic viability.
Effects of exogenous double-stranded RNA on the basonuclin gene expression in mouse oocytes
TLDR
DsRNA could inhibit the expression of the cognate gene in oocytes at both mRNA and protein levels, and offered a new way to study the function of basonuclin in the early stage of oogenesis by infection of primordial oocytes with the plasmid expressing hairpin-style b Masonuclin dsRNA.
A genetic link between co-suppression and RNA interference in C. elegans
TLDR
It is reported that mutants of C. elegans that are defective in transposon silencing and RNA interference are in addition resistant to co-suppression, indicating that RNA interference and co-Suppression in C. elegans may be mediated at least in part by the same molecular machinery, possibly through RNA-guided degradation of messenger RNA molecules.
RNA Interference and Small Interfering RNAs
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    Chembiochem : a European journal of chemical biology
  • 2001
TLDR
This minireview will highlight recent advances in understanding the molecular mechanism of RNAi and its biological function and identify components of the RNAi machinery required for posttranscriptional silencing by cosuppression.
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