Potassium transport inSchizosaccharomyces pombe

@article{Heyer2008PotassiumTI,
  title={Potassium transport inSchizosaccharomyces pombe},
  author={M. Heyer and H. Lichtenberg-Frat{\'e} and J. Reid and M. Höfer},
  journal={Folia Microbiologica},
  year={2008},
  volume={42},
  pages={227-229}
}
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K+-Fluxes through plasma membrane ofSchizosaccharomyces pombe as measured by whole-cell patch clamp
TLDR
The patch damp technique makes it possible to precisely measure the current through single channels and/or through the whole plasma membrane of a single cell, as a function of applied voltage to obtain an I/U curve. Expand

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Starved yeast cells show a negligible transport of amino acids and other substrates; Horfik et al. [1], found that these cells require a preincubation with glucose to recover their transportExpand
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The overall properties of the channel suggest that it functions in balancing charge movements during secondary active transport in Saccharomyces, particularly with varied membrane voltage and cytoplasmic calcium concentration. Expand
The SpTRK Gene Encodes a Potassium-specific Transport Protein TKHp in Schizosaccharomyces pombe
TLDR
The SpTRK gene that encodes a protein of 833 amino acids, the predicted structure contains 12 putative membrane-spanning domains and resembles various high- and low-affinity systems for K+ transport in yeasts and plants, and is a potassium-specific transporter. Expand
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Values for the cytoplasmic pHs of yeast cells that were higher than those reported previously were obtained, probably because this fluorescent indicator did not seem to penetrate into the cell vacuole. Expand
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Yeast strains which grow poorly on submillimolar K+ due to the deletion of two K(+)-transporter genes are in fact missing a prominent K+ inward current present in wild-type cells, to facilitate structure-function studies of any electrogenic or electrophoretic ion transporters which can be expressed in the plasma membrane of that organism. Expand
TRK2 is not a low-affinity potassium transporter in Saccharomyces cerevisiae
TLDR
The existence of a unique K+ uptake system composed of several proteins is discussed, which is consistent with the different levels of activity of the moderate-affinity Rb+ influx, and trk1 delta TRK2 cells grow slowly in micromolar K+, trk 1 delta TRk2D cells grow rapidly, andtrk1Delta trk2 delta cells fail to grow. Expand
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An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator, and results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. Expand
TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae.
We identified a 180-kilodalton plasma membrane protein in Saccharomyces cerevisiae required for high-affinity transport (uptake) of potassium. The gene that encodes this putative potassiumExpand