Posttranslational sulfation of factor V is required for efficient thrombin cleavage and activation and for full procoagulant activity.

@article{Pittman1994PosttranslationalSO,
  title={Posttranslational sulfation of factor V is required for efficient thrombin cleavage and activation and for full procoagulant activity.},
  author={Debra D. Pittman and Kathy N Tomkinson and D A Michnick and U Selighsohn and Randal J Kaufman},
  journal={Biochemistry},
  year={1994},
  volume={33 22},
  pages={6952-9}
}
Factor VIII and factor V function as cofactors in the blood coagulation cascade to accelerate the rate of activation of factor X and prothrombin, respectively. Both cofactors require proteolytic activation by either activated factor X or thrombin for functional activity. Human factor VIII and factor V expressed in mammalian cells are both modified by posttranslational sulfation of tyrosine residues. In the present study, the posttranslational addition of sulfate in factor V expressed in… CONTINUE READING