Posttranslational modification of tubulin by palmitoylation: II. Identification of sites of palmitoylation.

@article{Ozols1997PosttranslationalMO,
  title={Posttranslational modification of tubulin by palmitoylation: II. Identification of sites of palmitoylation.},
  author={Juris Ozols and Joan McIntyre Caron},
  journal={Molecular biology of the cell},
  year={1997},
  volume={8 4},
  pages={637-45}
}
As shown in the companion article, tubulin is posttranslationally modified in vivo by palmitoylation. Our goal in this study was to identify the palmitoylation sites by protein structure analysis. To obtain quantities of palmitoylated tubulin required for this analysis, a cell-free system for enzymatic [3H]palmitoylation was developed and characterized in our companion article. We then developed a methodology to examine directly the palmitoylation of all 451 amino acids of alpha-tubulin. 3H… CONTINUE READING