Postsynthetic Domain Assembly with NpuDnaE and SspDnaB Split Inteins.

Abstract

Inteins are protein segments embedded in frame within a precursor sequence that catalyze a self-excision reaction and ligate the flanking sequences with a standard peptide bond. Split inteins are expressed as two separate polypeptide fragments and trans-splice upon subunit association. Split inteins have found use in biotechnology applications but their use… (More)
DOI: 10.1007/s12010-015-1802-0

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