Postreplicative chromatin assembly by Drosophila and human chromatin assembly factor 1.

@article{Kamakaka1996PostreplicativeCA,
  title={Postreplicative chromatin assembly by Drosophila and human chromatin assembly factor 1.},
  author={Rohinton T. Kamakaka and Michael Bulger and Paul D Kaufman and Bruce W. Stillman and James T. Kadonaga},
  journal={Molecular and cellular biology},
  year={1996},
  volume={16 3},
  pages={810-7}
}
To study the relationship between DNA replication and chromatin assembly, we have purified a factor termed Drosophila chromatin assembly factor 1 (dCAF-1) to approximately 50% homogeneity from a nuclear extract derived from embryos. dCAF-1 appears to consist of four polypeptides with molecular masses of 180, 105, 75, and 55 kDa. dCAF-1 preferentially mediates chromatin assembly of newly replicated DNA relative to unreplicated DNA during T-antigen-dependent simian virus 40 DNA replication in… CONTINUE READING

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