Postnatal development of dye‐coupling among astrocytes in rat visual cortex

@article{Binmller1992PostnatalDO,
  title={Postnatal development of dye‐coupling among astrocytes in rat visual cortex},
  author={F J Binm{\"o}ller and Christian M. M{\"u}ller},
  journal={Glia},
  year={1992},
  volume={6}
}
Intercellular coupling among astrocytes was studied in rat visual cortex slices from animals aged 1 week to 4 months. Cell coupling via gap junctions was determined by the dye spread of the low molecular weight dye Lucifer Yellow CH injected into electrophysiologically identified cells to adjacent cells. Coupling among glial cells was first detected at postnatal day 11 and was thereafter consistently observed until adulthood. Dye spread was observed up to 300 μm radially from the injected cell… 
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It is found that passive astrocytes, which predominate after 3 wk postnatally, have much lower membrane resistances (Rm) and are more frequently dye coupled and to more cells, than outwardly and variably rectifying astroCytes that predominated in early postnatal development.
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TLDR
Combination of dye-coupling experiments performed with biocytin and immunostaining with specific cell markers demonstrated that astrocytic networks do not involve neurons, oligodendrocytes or NG2 cells, and indicated a preferential orientation of coupling inside the barrels resulting from subpopulations of astROcytes with different coupling properties that contribute to shaping astroCytic networks.
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Intercellular communication mediated by gap junctions was investigated during oligodendrocyte differentiation in primary and secondary cell cultures from newborn and adult rats, finding a developmental pattern comparable to homotypic dye‐coupling between oligodendedrocytes.
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References

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