Postinoculation protozoan establishment and association patterns of methanogenic archaea in the ovine rumen.

@article{OheneAdjei2007PostinoculationPE,
  title={Postinoculation protozoan establishment and association patterns of methanogenic archaea in the ovine rumen.},
  author={Samuel Ohene-Adjei and Ronald M. Teather and Michael Lyazi Ivan and Robert J. Forster},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 14},
  pages={4609-18}
}
Association patterns between archaea and rumen protozoa were evaluated by analyzing archaeal 16S rRNA gene clone libraries from ovine rumen inoculated with different protozoa. Five protozoan inoculation treatments, fauna free (negative control), holotrich and cellulolytic protozoa, Isotricha and Dasytricha spp., Entodinium spp., and total fauna (type A) were tested. We used denaturing gradient gel electrophoresis, quantitative PCR, and phylogenetic analysis to evaluate the impact of the… CONTINUE READING
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