Postharvest application of a novel chitinase cloned from Metschnikowia fructicola and overexpressed in Pichia pastoris to control brown rot of peaches.

@article{Banani2015PostharvestAO,
  title={Postharvest application of a novel chitinase cloned from Metschnikowia fructicola and overexpressed in Pichia pastoris to control brown rot of peaches.},
  author={Houda Banani and Davide Spadaro and Dianpeng Zhang and Slavica Mati{\'c} and A. M. Garibaldi and M. L. Gullino},
  journal={International journal of food microbiology},
  year={2015},
  volume={199},
  pages={54-61}
}
Metschnikowia fructicola strain AP47 is a yeast antagonist against postharvest pathogens of fruits. The yeast was able to produce chitinase enzymes in the presence of pathogen cell wall. A novel chitinase gene MfChi (GenBank accession number HQ113461) was amplified from the genomic DNA of Metschnikowia fructicola AP47. Sequence analysis showed lack of introns, an open reading frame (ORF) of 1098 bp encoding a 365 amino acid protein with a calculated molecular weight of 40.9 kDa and a predicted… CONTINUE READING
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