Post-vitrification survival and in vitro maturation rate of buffalo (Bubalus bubalis) oocytes: effect of ethylene glycol concentration and exposure time.

@article{Dhali2000PostvitrificationSA,
  title={Post-vitrification survival and in vitro maturation rate of buffalo (Bubalus bubalis) oocytes: effect of ethylene glycol concentration and exposure time.},
  author={Arindam Dhali and R. S. Manik and Shishir Kumar Das and Suresh Kumar Singla and Prabhat Palta},
  journal={Animal reproduction science},
  year={2000},
  volume={63 3-4},
  pages={159-65}
}
The present study was undertaken to investigate the effects of ethylene glycol concentration and time of exposure to equilibration solution on the post-thaw morphological appearance and the in vitro maturation rate of buffalo oocytes. Vitrification solution-I (VS-I) consisted of 4.5M ethylene glycol (EG), 3.4M dimethyl sulphoxide, 5. 56mM glucose, 0.33mM sodium pyruvate and 0.4% w/v bovine serum albumin in Dulbecco's phosphate buffered saline (DPBS), whereas vitrification solution-II (VS-II… CONTINUE READING