Post-translational modifications of protein biopharmaceuticals.

@article{Walsh2009PosttranslationalMO,
  title={Post-translational modifications of protein biopharmaceuticals.},
  author={Gary Walsh},
  journal={Drug discovery today},
  year={2009},
  volume={15 17-18},
  pages={
          773-80
        }
}
  • G. Walsh
  • Published 15 April 2009
  • Biology
  • Drug discovery today

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References

SHOWING 1-10 OF 51 REFERENCES

Post-translational modifications in the context of therapeutic proteins

A better understanding of the relationship between structure and function is understood for many PTMs but remains incomplete for others, particularly in the case of complex PTMs, such as glycosylation.

Glycome profiling using modern glycomics technology: technical aspects and applications

This review highlights the evolution of glycan profiling tools in the past 5 years and focuses on capillary electrophoresis, liquid chromatography, mass spectrometry and lectin microarrays.

Glycoengineering: the effect of glycosylation on the properties of therapeutic proteins.

The discovery of darbepoetin alfa (DA), a hyperglycosylated analogue of erythropoietin that contains two additional N-linked carbohydrates, a threefold increase in serum half-life and increased in vivo activity compared to recombinant human ery Anthropo-EPO, is discussed.

Production and glycosylation of plant-made pharmaceuticals: the antibodies as a challenge.

Transgenic plants are focused on as a promising system for the scale-up and processing of plant-made pharmaceuticals and the advantages and limitations induced by glycosylation of plants-made antibodies for human therapy are pointed out.

Post-translational Modifications Required for Coagulation Factor Secretion and Function

The role of post-translational modification in protein function investigators have studied proteins treated with chemicals or enzymes to remove modifications, proteins synthesized in the presence of inhibitors of specific modification reactions, proteins expressed in either different cell types or in cell mutants with defects in specific enzymatic machinery required to perform modifications, or proteins engineered through recombinant DNA technology that contain mutations that prevent modifications.

The production of biopharmaceuticals in plant systems.

Glycosylation as a strategy to improve antibody-based therapeutics

Current knowledge of methods and avenues for their exploitation in the clinic are summarized, which allow the production of rMAbs bearing pre-selected oligosaccharides — glycoforms — to provide maximum efficacy for a given disease indication.

Post‐translational modifications in proteins involved in blood coagulation

The post-translational modifications that occur in blood coagulation proteins are described and, where known, their functional implications are described.
...