Post-translational maturation of natural and drug-induced missorted phytohemagglutinin.

@article{DenmatOuisse1999PosttranslationalMO,
  title={Post-translational maturation of natural and drug-induced missorted phytohemagglutinin.},
  author={Denmat-Ouisse and Faye and Gomord},
  journal={Plant physiology and biochemistry : PPB},
  year={1999},
  volume={37 11},
  pages={849-858}
}
The bean lectin phytohemagglutinin (PHA) was expressed in transgenic suspension-cultured BY-2 tobacco cells simultaneously with another recombinant vacuolar protein, the sweet potato sporamin. In contrast to previous observations in different transgenic plant systems when expressed in BY-2 tobacco cells, phytohemagglutinin is mostly but not exclusively targeted to the vacuole. Indeed, a small amount of recombinant phytohemagglutinin is secreted into the culture medium of tobacco cells… CONTINUE READING