Post-transcriptional regulation of inducible nitric oxide synthase mRNA in murine macrophages by doxycycline and chemically modified tetracyclines.

@article{Amin1997PosttranscriptionalRO,
  title={Post-transcriptional regulation of inducible nitric oxide synthase mRNA in murine macrophages by doxycycline and chemically modified tetracyclines.},
  author={Ashok R. Amin and Rajesh N. Patel and Geeta D. Thakker and Charles J Lowenstein and Mukundan G. Attur and Steven B. Abramson},
  journal={FEBS letters},
  year={1997},
  volume={410 2-3},
  pages={259-64}
}
Chemically modified tetracyclines [CMT-3 (IC50 approximately 6-13 microM = approximately 2.5-5 microg/ml) and CMT-8 (IC50 approximately 26 microM = 10 microg/ml), but not CMT-1, -2 or -5], which lack anti-microbial activity, inhibited nitrite production in LPS-stimulated macrophages. Unlike competitive inhibitors of L-arginine which inhibited the specific activity of inducible nitric oxide synthase (iNOS) in cell-free extracts, CMTs exerted no such direct effect on the enzyme. CMTs could… CONTINUE READING